Journal
JOURNAL OF CONTROLLED RELEASE
Volume 133, Issue 3, Pages 214-220Publisher
ELSEVIER
DOI: 10.1016/j.jconrel.2008.10.003
Keywords
Ultradeformable liposomes; Delivery vehicle; Skin; Melanocytes; siRNA
Funding
- Fund for Scientific Research-Flanders (Belgium)
- Ghent University, Belgium [01 J04207]
- Pierre Fabre Research Institute (Centre d'Etudes et de Recherche Sur la Peau et les Epitheliums de Revetements, Toulouse, France)
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The aim of this work was to develop a system that can deliver siRNA into cells present in the human epidermis. More specifically, we wanted to block the expression of a specific Myosin Va exon F containing isoform that is physiologically involved in melanosome transport in human melanocytes. Therefore, we prepared and investigated the capacity of ultradeformable cationic liposomes (UCLs) to deliver siRNA in hard-to-transfect human primary melanocytes. UCLs were formulated from different w:w ratios (6:1, 8:1 and 10:1) of the cationic lipid 1,2-dioleoyl-3-trimethylammonium propane (DOTAP) and the edge activator sodium cholate. Subsequently, UCL/siRNA complexes were prepared and their particle size, surface charge, deformability, cytotoxicity, transfection efficiency and long-term stability were tested. The best results were obtained with UCLs composed of a DOTAP/NaChol ratio of 6:1 (w:w) which are promising for future in vivo experiments. (C) 2008 Elsevier B.V. All rights reserved.
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