Journal
JOURNAL OF COMPUTER-AIDED MOLECULAR DESIGN
Volume 25, Issue 8, Pages 743-752Publisher
SPRINGER
DOI: 10.1007/s10822-011-9455-8
Keywords
Molecular modeling; High throughput screening; Computational biology; Pharmacology; Drug discovery
Categories
Funding
- Progetti di Ricerca di Interesse Nazionale COFIN-PRIN
- Ministero dell'Istruzione dell'Universita e della Ricerca [GPR17]
- Cariplo Foundation [2008.2907]
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GPR17, a previously orphan receptor responding to both uracil nucleotides and cysteinyl-leukotrienes, has been proposed as a novel promising target for human neurodegenerative diseases. Here, in order to specifically identify novel potent ligands of GPR17, we first modeled in silico the receptor by using a multiple template approach, in which extracellular loops of the receptor, quite complex to treat, were modeled making reference to the most similar parts of all the class-A GPCRs crystallized so far. A high-throughput virtual screening exploration of GPR17 binding site with more than 130,000 lead-like compounds was then applied, followed by the wet functional and pharmacological validation of the top-scoring chemical structures. This approach revealed successful for the proposed aim, and allowed us to identify five agonists or partial agonists with very diverse chemical structure. None of these compounds could have been expected 'a priori' to act on a GPCR, and all of them behaved as much more potent ligands than GPR17 endogenous activators.
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