4.4 Article

Improved pKa Prediction: Combining Empirical and Semimicroscopic Methods

Journal

JOURNAL OF COMPUTATIONAL CHEMISTRY
Volume 29, Issue 15, Pages 2575-2581

Publisher

WILEY
DOI: 10.1002/jcc.20999

Keywords

pK(a); electrostatic; empirical; PROPKA; Karlsberg; consensus

Ask authors/readers for more resources

Using three different methods we tried to compute 171 experimentally known pK(a) values of ionizable residues front 15 different proteins and compared the accuracies of computed pK(a) values in terms of the root mean square deviation (RMSD) from experiment. One method is based on a continuum electrostatic model of the protein including conformational flexibility (KBPLUS). The others are empirical approaches with PROPKA deploying physically motivated energy terms with adjustable parameters and PKAcal using an empirical function with no physical basis. PROPKA reproduced the pK(a) values with highest overall accuracy. Differentiating the data set into weakly and strongly shifted experimental pK(a) values, however, we found that PROPKA's accuracy is better if the pK(a) values are weakly shifted but on equal footing with that of KBPLUS for more strongly shifted values. On the other hand, PKAcal reproduces strongly shifted pK(a) values badly but weakly shifted values with the same accuracy as PROPKA. We tested different consensus approaches Combining data front all three methods to find a general procedure for most accurate pK(a) predictions. In most of the cases we found that the consensus approach reproduced experimental data with better accuracy than any of the individual methods alone. (C) 2008 Wiley Periodicals, Inc. J Comput Chem 29: 2575-2581, 2008

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.4
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available