Journal
JOURNAL OF COMPARATIVE PATHOLOGY
Volume 146, Issue 2-3, Pages 243-252Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.jcpa.2011.06.005
Keywords
cytokine mRNA; foot-and-mouth disease; pharyngeal epithelium
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Funding
- Technical University of Denmark (DTU)
- Research School for Animal Production and Health at the Faculty of Life Sciences, Copenhagen University
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A novel technique of endoscopical collection of small tissue samples was used to obtain sequential tissue samples from the dorsal soft palate (DSP) of individual cattle infected with foot-and-mouth disease virus (FMDV) at different phases of the infection. Levels of mRNA encoding interferon (IFN)-alpha and IFN-beta as well as tumour necrosis factor (TNF)-alpha were measured in these samples by quantitative reverse transcriptase polymerase chain reaction. Expression of IFN-beta mRNA was significantly down-regulated in the biopsy samples harvested during the acute phase of infection, while there was no statistically significant effect on the expression of IFN-alpha mRNA compared with baseline levels. In contrast, the mRNA encoding TNF-alpha was significantly up-regulated in samples collected during both acute and late (>28 days post infection) phases of infection. There were also significantly higher levels of TNF-alpha mRNA expressed in samples derived from animals that were identified subsequently as persistently infected FMDV-carriers. It was concluded that there was a significant difference in the host-response in the DSP of calves that were identified as persistently infected, subclinical carriers of FMDV. (C) 2011 Elsevier Ltd. All rights reserved.
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