Journal
JOURNAL OF COMPARATIVE NEUROLOGY
Volume 522, Issue 14, Pages 3194-3207Publisher
WILEY-BLACKWELL
DOI: 10.1002/cne.23590
Keywords
oligodendrocytes; gliogenesis; ependyma
Categories
Funding
- VEGA [1/0967/12, 1/0322/11, 1/0391/10]
- VVGS [PF-2013-88]
- NEXO (Network of Excellence in Oncology) [007/20092.1/OPVaV]
- Structural Funds of the E.U. [ITMS 26220120058]
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Two waves of oligodendrogenesis in the ventricular zone of the spinal cord (SC-VZ) during rat development, which take place between embryonic days 14 and 18 (E14-E18) and E20-E21, have been described. In the VZ of the brain, unlike the SC-VZ, a third wave of oligodendrogenesis occurs during the first weeks of postnatal development. Using immunofluorescence staining of intact rat SC tissue, we noticed the presence of small numbers of Olig2(+)/Sox-10(+) cells inside the lining of the central canal (CC) during postnatal development and adulthood. Olig2(+)/Sox-10(+) cells appeared inside the lining of the CC shortly after birth, and their number reached a maximum of approximately 0.65 +/- 0.14 cell/40-mu m section during the second postnatal week. After the latter development, the number of Olig2(+)/Sox-10(+) cells decreased to 0.21 +/- 0.07 (P36) and 0.18 +/- 0.1 cell/section (P120). At P21, Olig2(+)/Sox10(+) cells inside the CC lining started to express other oligodendroglial markers such as CNPase, RIP, and APC. Olig2(+)/Sox-10(+) cells usually did not proliferate inside the CC lining and were only rarely found to be immunoreactive against oligodendrocyte progenitor markers such as NG2 or PDGFRa. Using 5-bromo-2-deoxyuridine administration at P2, P11, P22, or P120-P125, we revealed that these cells arose in the CC lining during postnatal development and adulthood. Our findings confirmed that the CC lining is the source of a small number of cells with an oligodendroglial phenotype during postnatal development and adulthood in the SC of intact rats. (C) 2014 Wiley Periodicals, Inc.
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