Journal
JOURNAL OF COLLOID AND INTERFACE SCIENCE
Volume 416, Issue -, Pages 252-257Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jcis.2013.11.008
Keywords
Layer-by-layer; pH sensing; Polyelectrolyte microcapsule; Cell uptake; Ratiometric measurement
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Funding
- Natural Science Foundation of China [21174130, 51120135001]
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Encapsulation of pH sensitive fluorophores as reporting molecules provides a powerful approach to visualize the transportation of multilayer capsules. In this study, two pH sensitive dyes (fluorescein and oregon green) and one pH insensitive dye (rhodamine B) were simultaneously labeled on the microcapsules to fabricate ratiometric pH sensors. The fluorescence of the triple-labeled microcapsule sensors was robust and nearly independent of other intracellular species. With a dynamic pH measurement range of 3.3-6.5, the microcapsules can report their localized pH at a real time. Cell culture experiments showed that the microcapsules could be internalized by RAW 246.7 cells naturally and finally accumulated in acidic organelles with a pH value of 5.08 +/- 0.59 (mean +/- s.d.; n = 162). (C) 2013 Elsevier Inc. All rights reserved.
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