Journal
JOURNAL OF COLLOID AND INTERFACE SCIENCE
Volume 398, Issue -, Pages 82-87Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jcis.2013.02.001
Keywords
Spherical brushes; Poly(acrylic acid) (PAA); RAFT polymerization; Protein immobilization
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Funding
- NSF of China [21075082]
- 863 High Tech Program [2012AA020103]
- Shanghai Nano program [11nm0505600]
- Science and Technology Commission of the Xuhui District [RCT 201007]
- SJTU [YG2012ZD03]
- China Postdoctoral Science Foundation [2012M511088]
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Spherical poly(acrylic acid) brushes were prepared via a facile RAFT polymerization route from silica nanoparticles (SiO2@PAAs). A silane functionalized RAFT chain transfer agent was designed and synthesized by a one-step reaction, and then immobilized onto silica nanoparticles (SiNPs) through its R group to afford RAFT polymerization. Key structural parameters and contents of carboxyl groups of SiO2@PAAs were thoroughly characterized by transmission electron microscopy, dynamic light scattering, gel permeation chromatography, thermogravimetric analysis and conductometric titration. The SiO2@PAAs exhibit excellent dispersity, tunable brush thicknesses (14.6-68.8 nm) and abundant carboxyl groups (0.82-2.37 mmol/g). An ultra-high protein immobilization capacity (2600 mu g streptavidin/mg SiO2@PAAs) was realized by virtue of its rich carboxyl groups and spherical brush structure, which opens up new possibilities for biomedical applications. (C) 2013 Elsevier Inc. All rights reserved.
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