Journal
JOURNAL OF COLLOID AND INTERFACE SCIENCE
Volume 374, Issue -, Pages 206-217Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jcis.2012.01.053
Keywords
DODAB; Monoolein; Pyrene; Differential scanning calorimetry; Langmuir monolayers; Fluorescence spectroscopy; Light microscopy; Cryo-TEM
Categories
Funding
- FCT - Portugal
- COMPETE through CFUM [PEst-C/FIS/UI0607/2011 (F-COMP-01-0124-FEDER-022711)]
- Centro de Investigacao em Quimica-Univ. Porto (CIQUP)
- FCT [SFRH/BD/46968/2008]
- [PTDC/QUI-QUI/115212/2009]
- [PTDC/QUI/69795/2006]
- Fundação para a Ciência e a Tecnologia [PTDC/QUI/69795/2006, PTDC/QUI-QUI/115212/2009, SFRH/BD/46968/2008] Funding Source: FCT
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A recently described non-viral gene delivery system [dioctadecyldimethylammonium bromide (DODAB)/monoolein (MO)] has been studied in detail to improve knowledge on the interactions between lamellar (DODAB) and non-lamellar-forming (MO) lipids, as a means to enhance their final cell transfection efficiency. Indeed, the morphology, fluidity, and size of these cationic surfactant/neutral lipid mixtures play an important role in the ability of these systems to complex nucleic acids. The different techniques used in this work, namely dynamic light scattering (DLS), fluorescence spectroscopy, differential scanning calorimetry (DSC), cryogenic transmission electron microscopy (cryo-TEM), light microscopy (LM), and surface pressure-area isotherms, allowed fully characterization of the phase behavior and aggregate morphology of DODAB/MO mixtures at different molar ratios. Overall, the results indicate that the final morphology of DODAB/MO aggregates depends on the balance between the tendency of DODAB to form zero-curvature bilayer structures and the propensity of MO to form non-bilayer structures with negative curvature. These results also show that in the MO-rich region, an increase in temperature has a similar effect on aggregate morphology as an increase in MO concentration. (C) 2012 Elsevier Inc. All rights reserved.
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