Journal
JOURNAL OF COLLOID AND INTERFACE SCIENCE
Volume 364, Issue 1, Pages 133-139Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jcis.2011.08.030
Keywords
Langmuir monolayer; Plant sterols; Grazing Incidence X-ray Diffraction (GIXD); Brewster Angle Microscopy
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Funding
- European Regional Development Fund of the Polish Innovation Economy Operational Program [POIG.02.01.00-12-023/08]
- DOE Office of Basic Energy Sciences
- Los Alamos National Laboratory under DOE [DE-AC52-06NA25396.C]
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Although the influence of structurally modified sterols on artificial membranes has been intensively investigated, studies on the properties of stanols, which are saturated analogs of sterols, are very rare. Therefore, we have performed Grazing Incidence X-ray Diffraction (GIXD) experiments aimed at studying in-plane organization of a plant stanol-beta-sitostanol monolayer and its mixtures with 1,2-dipalmitoyl-sn-glycero-3-phosphocholine - DPPC at the air/water interface. The collected GIXD data, resulting in-plane parameters and BAM images provide information on molecular organization and in-plane ordering of the investigated films. It was found that the lateral organization of beta-sitostanol/DPPC monolayers depends on their composition. The oblique structure of the in-plane lattice of tilted hydrophobic region of molecules, found for DPPC film, is maintained at 10 mol% of stanol in the system. However, at 30 and 90 mol% of stanol in the mixture, the arrangement of molecules is hexagonal and they are oriented perpendicularly to the interface. With the addition of stand the extend of the in-plane order of the monolayers decreases. Moreover, in mixtures the ordered domains consist of both monolayer's components. Additionally, p-sitostanol film is of similar in-plane organization as the corresponding sterol monolayer (beta-sitosterol) and stanol induces condensing effect on DPPC. (C) 2011 Elsevier Inc. All rights reserved.
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