Journal
JOURNAL OF COLLOID AND INTERFACE SCIENCE
Volume 334, Issue 2, Pages 183-187Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jcis.2009.02.043
Keywords
Zinc oxide nanoparticles; Photoelectrochemical system; Glucose oxidase; Biosensor
Categories
Funding
- National Science Foundation of China [60602006, 60736001]
- Knowledge Innovation Program of the Chinese Academy of Sciences [TYF0808]
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Nanosized semiconductor crystals can increase efficiency of photochemical reactions and greatly improve the catalytic activity of enzymes to generate novel photoelectrochemical systems. In this work, glucose oxidase (GOx)/zinc oxide (ZnO) is selected as a model system to assess the photovoltaic effect of semiconductor nanoparticles on the enzyme electrode. UV-spectrum and circular dichroism (CD) results show that the structure of GOx is preserved after Conjugation with ZnO nanoparticles. The current response of the enzyme electrode containing ZnO nanoparticles increases from 0.82 to 21 mu A cm(-2) in the solution of 10 mM beta-D-glucose. Furthermore, after irradiating the enzyme electrode with UV light for 2 h, the current response can be increased nearly 30% and the detection limit can be lowered about two orders compared with the catalytic reactions in the dark, which indicate that a technique to fabricate a novel photocontrolled enzyme-based biosensor may be developed. (C) 2009 Elsevier Inc. All Fights reserved.
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