Journal
JOURNAL OF CLINICAL VIROLOGY
Volume 56, Issue 3, Pages 250-254Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jcv.2012.11.020
Keywords
Frequency; Human rhinovirus; Stool samples; RT-PCR
Categories
Funding
- Juvenile Diabetes Research Foundation
- Sohlberg Foundation
- Academy of Finland (SHOK)
- Tampere University Hospital
- PEVNET project (European Seventh Framework Programme) [GA-261441-PEVNET]
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Background: Human rhinoviruses (HRVs) are common causes of viral respiratory infections. They have been widely studied in respiratory samples in hospital patient series but only a few studies have been performed to assess their occurrence in other sample types and their circulation in healthy children background population. Objectives: To analyze the frequency of HRVs in the background population in Finland by screening HRV RNA from stool samples longitudinally collected in a cohort of young children. Study design: Altogether 4184 stool samples were collected regularly from a cohort of children who were observed from birth. Samples were screened for the presence of RNA of HRVs using RT-PCR. HRV specific sequences were identified by sequencing the VP1 or VP4/VP2 coding region. Virus isolation was performed using four different cell lines and the result was confirmed by real time PCR. Results: A total of 9% of the stool samples were positive for HRV RNA. Sequence analysis indicated that the most prevalent species was HRV-A, and the most prevalent serotype was HRV61. HRV-B and HRV-C species were also detected. One of the six tested rhinovirus positive samples retained its infectivity and was able to grow in RD and GMK cells. Conclusions: Our study shows that HRVs are frequently detected in the stool samples from the population of young children. We also show that HRV-C, which can cause severe illnesses in children, is commonly circulating in young children in Finland. (C) 2012 Elsevier B. V. All rights reserved.
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