Journal
JOURNAL OF CLINICAL VIROLOGY
Volume 46, Issue -, Pages S68-S72Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jcv.2009.07.003
Keywords
Virus; Vaccine; T cells; Antibody; Protection; Immunotherapy
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In spite of numerous attempts at successful licensure, a human cytomegalovirus (HCMV) vaccine formulation remains elusive. To overcome the limitations of previous strategies, we have recently developed a novel chimeric vaccine which allows induction of both humoral and cellular immune response following a single vaccination. This vaccine includes the extracellular domain of HCMV-encoded glycoprotein-B (gB) covalently linked to multiple HLA class I- and class II-restricted T-cell epitopes from multiple HCMV antigens as a contiguous polypeptide in a replication-deficient adenoviral vector Ad5/F35 (referred to as Ad-gBCMVpoly). Immunisation with Ad-gBCMVpoly consistently generated strong gB-specific neutralizing antibody and a broad range of HCMV-specific pluripotent CD8(+) and CD4(+) T-cells. This immunity suppressed infection with recombinant vaccinia virus encoding HCMV antigens. Furthermore, in vitro stimulation with Ad-gBCMVpoly rapidly expanded multiple antigen-specific human CD8(+) and CD4(+) T-cells from healthy virus carriers. Here we discuss the advantages of the Ad-gBCMVpoly vaccine and its potential application in different clinical settings. (c) 2009 Elsevier B.V. All rights reserved.
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