Journal
JOURNAL OF CLINICAL VIROLOGY
Volume 43, Issue 3, Pages 260-265Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jcv.2008.07.018
Keywords
Epstein-Barr virus; T-cell prolymphocytic leukemia; Infection
Categories
Funding
- Leukemia and Lymphoma Society of America [CA072510, CA091792]
- NIDCR [DE014136-04, 017338]
- Leukemia and Lymphoma Society of America
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Background: Epstein-Barr virus (EBV) is closely associated with the development of a number of tumors. During latent infection, EBV continuously expresses a number of viral genes which are essential for cell transformation and maintenance of the malignant phenotype of EBV-related tumors. There has been no previous link between EBV and T-cell prolymphocytic leukemia (T-PLL), a distinctive form of leukemia derived from T-cells at an intermediate stage of differentiation between a cortical thymocyte and a mature peripheral blood T-cell. Objective: To determine if EBV was present in the T-PLL cells collected. Study design: T-PLL cells were isolated from the peripheral blood of a patient diagnosed with T-PILL and Continuously Cultured for about I year. The existence of EBV in these cells was detected Using multiple strategies including PCR, Western blotting, immunofluorescent assay and flow cytometry analysis. Results: The EBV genome was present in these T-PLL cells by PCR analysis across multiple sites in the viral genome. In addition, these T-PLL cells expressed a number of EBV latent antigens. The EBV oncoproteins LMP1, EBNA1 and EBNA3C were expressed in the majority of the infected cells. Conclusion: This report suggests a potential link between EBV infection and T-PLL and provides new information about the potential contribution of EBV in the initiation or maintenance of T-PLL. (C) 2008 Elsevier B.V. All rights reserved.
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