4.7 Article

Performance of Panfungal-and Specific-PCR-Based Procedures for Etiological Diagnosis of Invasive Fungal Diseases on Tissue Biopsy Specimens with Proven Infection: a 7-Year Retrospective Analysis from a Reference Laboratory

Journal

JOURNAL OF CLINICAL MICROBIOLOGY
Volume 52, Issue 5, Pages 1737-1740

Publisher

AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.00328-14

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Funding

  1. Fundacion Mutua Madrile [PI11/00412]
  2. Instituto de Salud Carlos III
  3. Red Espa ola de Investigacion en Patolog a Infecciosa (REIPI) [1022/07_1]
  4. European Development Regional Fund
  5. Spanish Network for Research in Infectious Diseases [RD06/0008]

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A retrospective analysis of real-time PCR (RT-PCR) results for 151 biopsy samples obtained from 132 patients with proven invasive fungal diseases was performed. PCR-based techniques proved to be fast and sensitive and enabled definitive diagnosis in all cases studied, with detection of a total of 28 fungal species.

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