Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Volume 52, Issue 4, Pages 1217-1219Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.03106-13
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Funding
- National Health Research Institutes [IV-101-SP-18]
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PCR coupled with electrospray ionization mass spectrometry (PCR/ESI-MS) was compared with culture for pathogen detection in peritoneal dialysis (PD)-related peritonitis. Of 21 samples of PD effluent, PCR/ESI-MS identified microorganisms in 18 (86%) samples, including Mycobacterium tuberculosis in 1 culture-negative sample. Of 15 double-positive samples, PCR/ESI-MS and culture reached levels of agreement of 100% (15/15) and 87.5% (7/8) at the genus and species levels, respectively. PCR/ESI-MS can be used for rapid pathogen detection in PD-related peritonitis.
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