Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Volume 51, Issue 6, Pages 1920-1923Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.00064-13
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Funding
- National Natural Science Foundation of China [31000549]
- Shanghai Key Laboratory of Molecular Medical Mycology Fund [20110001]
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A singleplex PCR assay using a single primer pair targeting the putative sugar transporter gene was developed here to distinguish Cryptococcus neoformans var. grubii, Cryptococcus neoformans var. neoformans, and Cryptococcus gattii according to the distinct size of the amplicon. The interspecies and intravarietal hybrids were also characterized on the basis of distinct combined profiles of amplicons. This PCR assay is a rapid, simple, and reliable approach suitable for laboratory diagnoses and large-scale epidemiologic studies.
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