4.7 Article

Development of a Loop-Mediated Isothermal Amplification Assay Targeting the mpb64 Gene for Diagnosis of Intraocular Tuberculosis

Journal

JOURNAL OF CLINICAL MICROBIOLOGY
Volume 51, Issue 11, Pages 3839-3840

Publisher

AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.01386-13

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Funding

  1. Department of Science and Technology, government of India, New Delhi, India [SR/FT/LS-15/2011]
  2. Council of Scientific and Industrial Research (CSIR), Government of India, New Delhi, India

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A loop-mediated isothermal amplification (LAMP) assay targeting the mpb64 gene for the diagnosis of intraocular tuberculosis was highly specific (100%), sensitive (85.7%), rapid, and easy to perform. The LAMP assay can be an alternative to conventional PCR for the diagnosis of ocular tuberculosis in resource-limited settings.

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