Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Volume 50, Issue 10, Pages 3378-3380Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.01612-12
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Funding
- French Army department (Bourse DGA: Delegation Generale de l'Armement, Ministere de la Defense, Topic: Microbiology, infectious diseases)
- Clinical and Virological research (SFR-CAP sante) from the Medical University and School of Medicine of Reims, France [EA-4684]
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Standardized one-step real-time RT-PCR assay detected enterovirus RNA in cardiac biopsy samples from 4 of 20 patients suffering from idiopathic dilated cardiomyopathy (IDCM). The median viral load was 287 copies per microgram of total extracted nucleic acids, with positive-to negative-strand RNA ratios ranging from 2 to 20. These results demonstrate enterovirus persistence in the heart of IDCM patients, characterized by low viral loads and low positive- to negative-RNA ratios.
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