Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Volume 51, Issue 1, Pages 287-290Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.02365-12
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This study evaluates the accuracy of liquid chromatography-mass spectrometry (LC-MS) and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF) for detecting carbapenem hydrolytic activity among SPM-1-, GIM-1-, and GES-5-producing Pseudomonas aeruginosa isolates and OXA-143-, IMP-10-, and OXA-58-producing Acinetobacter baumannii isolates. Class A and B carbapenemase activities were rapidly detected by MALDI-TOF in a 2-h assay. However, an extended incubation time was necessary for detection of carbapenem-hydrolyzing class D beta-lactamase (CHDL) activity in Acinetobacter spp.
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