Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Volume 50, Issue 6, Pages 2126-2128Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.06829-11
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Funding
- INSERM [U851, U1052]
- French National Agency for Research on AIDS and Viral Hepatitis (ANRS)
- Hospices Civils de Lyon
- Assistance Publique-Hopitaux de Paris
- Universite Paris 13 (Interface INSERM)
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As for other chronic viral diseases, quantification of hepatitis delta virus (HDV) loads may be useful for patient management. We describe a one-step quantitative reverse transcription-PCR assay that is reliable and automatable and meets the regulatory authorities' standards for accurate quantification of the major HDV genotypes. It includes an internal control and uses in vitro-transcribed RNAs as standards. Its linearity range is 500 to 1.7 x 10(11) copies/ml, its sensitivity is around 150 copies/ml, its repeatability is around 15%, and its reproducibility is below 0.25 log(10) copies/ml.
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