4.7 Article

Development of a TaqMan Multiplex PCR Assay for Detection of Plasmid-Mediated AmpC β-Lactamase Genes

Journal

JOURNAL OF CLINICAL MICROBIOLOGY
Volume 50, Issue 11, Pages 3722-3725

Publisher

AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.02038-12

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Funding

  1. Becton, Dickinson GeneOhm Diagnostics

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A multiplex, real-time TaqMan assay was designed to identify clinical isolates carrying plasmid-mediated ampC genes. The specificity and sensitivity of this assay were 100% when testing characterized AmpC/non-AmpC-producing isolates and randomly selected clinical isolates. This is a rapid assay that can be performed in a clinical microbiology laboratory.

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