4.7 Article

Leishmania (Viannia) Species Identification on Clinical Samples from Cutaneous Leishmaniasis Patients in Peru: Assessment of a Molecular Stepwise Approach

Journal

JOURNAL OF CLINICAL MICROBIOLOGY
Volume 50, Issue 2, Pages 495-498

Publisher

AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.05061-11

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Funding

  1. European Community [INCO-CT2005-015407]
  2. Belgian Government [03-project 95502]
  3. Royal College of Physicians and Surgeons of Canada

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We present an algorithm based on three PCR assays for Leishmania (Viannia) species identification and assessed its performance using 70 specimens from Peruvian patients. The succession of the assayed targets can be ordered according to species prevalence. Sequential progression through the algorithm reduced the number of samples here studied by approximately 30% after each step.

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