New Diagnostic Microarray (Check-KPC ESBL) for Detection and Identification of Extended-Spectrum Beta-Lactamases in Highly Resistant Enterobacteriaceae

The performance of a microarray for the detection of extended-spectrum beta-lactamases was determined on a collection of 638 highly resistant members of the family Enterobacteriaceae collected from patients in 18 hospitals in The Netherlands. The microarray had a significantly higher specificity than the phenotypic assays. It also detects carbapenemases and characterizes the resistance genes, providing epidemiological insight.