Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Volume 48, Issue 10, Pages 3725-3728Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.01482-10
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Funding
- National Institute of Occupational Safety and Health, Centers for Disease Control and Prevention [2R01OH007364]
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An ATP-based biocide susceptibility assay for mycobacteria was developed by optimizing the cell lysis and assay conditions. Compared to the conventional agar plating method, the assay was rapid (1.5 h) and showed high sensitivity and specificity as determined by receiver operating characteristic (ROC) analysis. The test species, Mycobacterium immunogenum, M. chelonae, and M. abscessus, showed various susceptibilities to the glutaraldehyde- and isothiazolone-based test biocides.
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