4.7 Article

Spurious Amplification of a Plasmodium vivax Small-Subunit RNA Gene by Use of Primers Currently Used To Detect P. knowlesi

Journal

JOURNAL OF CLINICAL MICROBIOLOGY
Volume 47, Issue 12, Pages 4173-4175

Publisher

AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.00811-09

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Funding

  1. Wellcome Trust of Great Britain [080867/Z/06/Z]
  2. Wellcome Trust [080867/Z/06/Z] Funding Source: Wellcome Trust

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The PCR primers commonly used to detect Plasmodium knowlesi infections in humans were found to cross-react stochastically with P. vivax genomic DNA. A nested primer set that targets one of the P. knowlesi small-subunit rRNA genes was validated for specificity and for sensitivity of detection of <10 parasite genomes.

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