4.7 Article

Expanded Multilocus Sequence Typing for Burkholderia Species

Journal

JOURNAL OF CLINICAL MICROBIOLOGY
Volume 47, Issue 8, Pages 2607-2610

Publisher

AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.00770-09

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Funding

  1. U. S. Cystic Fibrosis Foundation
  2. United Kingdom Cystic Fibrosis Trust [PJ 535]
  3. Wellcome Trust [072853]

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PCR primers targeting loci in the current Burkholderia cepacia complex multilocus sequence typing scheme were redesigned to (i) more reliably amplify these loci from B. cepacia complex species, (ii) amplify these same loci from additional Burkholderia species, and (iii) enable the use of a single primer set per locus for both amplification and DNA sequencing.

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