Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Volume 48, Issue 2, Pages 612-614Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.01402-09
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- PneumoADIP
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We reformulated a multiplex PCR algorithm for serotyping of pneumococcal meningitis directly on cerebrospinal fluid (CSF). Compared to established methods on isolates, CSF-based PCR had at least 80% sensitivity and 100% specificity. In regional meningitis surveillance, CSF-based PCR increased the serotype information yield from 40% of cases (isolate testing) to 90%.
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