4.7 Article

Reverse Transcription-Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Hepatitis E Virus

Journal

JOURNAL OF CLINICAL MICROBIOLOGY
Volume 47, Issue 7, Pages 2304-2306

Publisher

AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.00498-09

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Funding

  1. State Scientific Research Institution for Public Welfare [BRF070307]

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The one-step single-tube betaine-free reverse transcription (RT)-loop-mediated isothermal amplification assay was developed for rapid diagnosis of hepatitis E virus. This assay amplified the target gene in less than 45 min (even as short as 20 min) under isothermal conditions at 63 C, and the sensitivity of this assay was 100-fold greater than that of RT-PCR. This assay demonstrated a detection limit of 0.045 fg (nine copies/reaction).

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