Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Volume 47, Issue 7, Pages 2252-2255Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.00033-09
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Funding
- NIH/NIAID [2 U54 AI057168]
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A rapid assay for eubacterial species identification is described using high-resolution melt analysis to characterize PCR products. Unique melt profiles generated from multiple hypervariable regions of the 16S rRNA gene for 100 clinically relevant bacterial pathogens, including category A and B biothreat agents and their surrogates, allowed highly specific species identification.
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