4.7 Article

Cross-Priming Amplification for Rapid Detection of Mycobacterium tuberculosis in Sputum Specimens

Journal

JOURNAL OF CLINICAL MICROBIOLOGY
Volume 47, Issue 3, Pages 845-847

Publisher

AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.01528-08

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Funding

  1. Key Project of Chinese National Programs [2008ZX100/03-010]
  2. Chinese National Programs 973 [2005CB 523102, 2006AA02Z423]
  3. National Institutes of Health [D43 TW007887]

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Cross-priming amplification (CPA) technology for tuberculosis diagnosis from sputum specimens was evaluated. The sensitivity of CPA from smear-and liquid culture-positive specimens was 96.9%, and that from smear-negative and liquid culture-positive specimens was 87.5%. The specificity of CPA in culture-negative specimens was 98.8%.

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