Journal
JOURNAL OF CLINICAL LABORATORY ANALYSIS
Volume 27, Issue 1, Pages 27-30Publisher
JOHN WILEY & SONS INC
DOI: 10.1002/jcla.21557
Keywords
human anti-mouse antibodies; IgM-capture EIA; false positivity; monoclonal IgG subclasses
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BackgroundApproximately 6% of sera positive in a dengue virus IgM-capture enzyme immunoassay (EIA) represent false-positives due to interaction between IgM and horseradish peroxidase (HRP)-labeled monoclonal antibody (MAb) 6B6C1 (IgG2a). To better understand this interaction, we assessed the reactivity of captured IgM from these sera with other HRP-labeled MAbs. J. Clin. Lab. Anal. 27:27-30, 2013. (c) 2012 Wiley Periodicals, Inc. MethodsFifty dengue IgM false-positive sera (recognizing 6B6C1) were evaluated for IgM reactivity with the HRP-labeled MAbs H3A4 (IgG2a), 53.8 (IgG2b), and IL-A2 (IgG1). The sera were also tested in an EIA for human anti-mouse antibody (HAMA). ResultsForty-three sera (86%) reacted with IgG2a MAb (H3A4). Most (31/43 = 72%) of these sera recognizing 6B6C1 and H3A4 also recognized the IgG2 MAb and/or the IgG1 MAb. In contrast, HAMA was increased in only 9 of 50 (18%) sera reacting with 6B6C1. ConclusionsIgM from most sera-binding IgG2a MAb 6B6C1 also binds another IgG2a MAb, suggesting that IgM-6B6C1 reactivity is not idiotype specific. In many cases, IgM recognizing 6B6C1 also binds MAbs of other IgG subclasses, but is negative in a HAMA assay. These findings indicate that samples positive in IgM-capture EIAs utilizing conjugated MAbs should always be retested in the absence of antigen to identify false-positive reactivity caused by direct IgM-MAb interaction. J. Clin. Lab. Anal. 00:1-4, 2012. (c) 2012 Wiley Periodicals, Inc.
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