4.5 Article

Development of a Bioassay System for Human Growth Hormone Determination with Close Correlation to Immunoassay

Journal

JOURNAL OF CLINICAL LABORATORY ANALYSIS
Volume 26, Issue 5, Pages 328-335

Publisher

WILEY
DOI: 10.1002/jcla.21527

Keywords

growth hormone; bioassay; BaF; GM cell line; high sensitivity; IRMA

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Serum growth hormone (GH) level is measured largely through immunoassays in clinical practice. However, a few cases with bioinactive and immunoreactive GH have also been reported. We describe here a new bioassay system for GH determination using the BaF/GM cell line, which proliferates in a dose-dependent manner on hGH addition; cell proliferation was blocked by anti-hGH antibody. This bioassay had the lowest detection limit (similar to 0.02 ng/ml) reported thus far and the highest specificity for GH. The bioassay results were compared with those of an immunoradiometric assay across 163 patient samples in various endocrine states. A close correlation (the ratio of bioactivity/immunoreactivity was 1.04 +/- 0.33, mean +/- SD) was observed between bioactivity and immunoreactivity in these samples. The newly developed system is a specific, sensitive, easy, and fast bioassay system for GH determination; we consider it useful for evaluating GH bioactivity in various endocrine states. J. Clin. Lab. Anal. 26:328-335, 2012. (c) 2012 Wiley Periodicals, Inc.

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