Journal
JOURNAL OF CLINICAL IMMUNOLOGY
Volume 28, Issue 5, Pages 464-472Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10875-008-9213-4
Keywords
Graves' disease; CD4(+)CD28(-) T cell; CD8(+)CD28(-) T cell; interferon (IFN)-gamma; peripheral blood mononuclear cells (PBMCs)
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Funding
- National Key Basic Research Program of China [2001 CB51003]
- National Natural Science Foundation of China [30471690]
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Background Peripheral blood CD4(+) and CD8(+) T-cell subsets lacking surface CD28 have been suggested to predispose patients to immune-mediated disorders. Materials and Methods To determine the role of CD28(-) T-cell subset in Graves' disease (GD), we characterized peripheral blood CD4(+)CD28(-) and CD8(+)CD28(-) T cell from early onset GD patients. Results and Discussion GD patients had significantly higher percentages of CD4(+)CD28(-) and CD8(+)CD28(-) T cells than did healthy donors. Both CD28(-) T cells expressed mostly CD45RO, suggesting that they are activated and/or are memory T cells. GD patient-derived CD4(+)CD28(-) and CD8(+)CD28(-) T cells produced more intracellular IFN-gamma than their counterparts from healthy donors. Furthermore, CD4(+)CD28(-) and CD8(+)CD28(-) T cells from GD patients with Graves' ophthalmopathy (GO) secreted higher level of intracellular IFN-gamma than those CD28(-) T cells from GD patients without GO. Retrospective analysis showed that the increased levels of CD4(+)CD28(-) T cells and their IFN-gamma-producing subgroups were positively correlated to the serum anti-thyrotropin receptor (TSHR) autoantibodies (TRAb). Our observations suggest that increased IFN-gamma-producing CD28(-) T cells in GD patients may play an important role in the pathogenesis of GD.
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