Journal
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
Volume 97, Issue 7, Pages E1182-E1186Publisher
ENDOCRINE SOC
DOI: 10.1210/jc.2011-2963
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Funding
- National Institutes of Health [DK-038765, DK-083562, PO1 HL-55782]
- T32 Training Grant UAB Obesity Training Program [HL-079888]
- Department of Veterans Affairs
- Gatorade Sports Science Institute
- UAB Center for Clinical and Translational Science [UL1 RR025777]
- Nutrition and Obesity Research Center [P30 DK56336]
- Diabetes Research and Training Center [P60 DK079626]
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Objective: The relationships among skeletal muscle lipid peroxidation, intramyocellular lipid content (IMCL), and insulin sensitivity were evaluated in nine insulin-sensitive (IS), 13 insulin-resistant (IR), and 10 adults with type 2 diabetes (T2DM). Design: Insulin sensitivity was assessed by hyperinsulinemic-euglycemic clamp [ glucose disposal rate (GDR)]. Lipid peroxidation was assessed by 4-hydroxynonenal (HNE)-protein adducts and general oxidative stress by protein carbonyl content. All patients were sedentary. Results: Protein-HNE adducts were elevated 1.6-fold in T2DM compared with IS adults, whereas IR showed intermediate levels of HNE-modified proteins. Protein-HNE adducts correlated with GDR, waist circumference, and body mass index. IMCL was increased by 4.0- and 1.9-fold in T2DM and IR patients, respectively, compared with IS, and was correlated with GDR and waist circumference but not BMI. Protein carbonyls were not different among groups and did not correlate with any of the measured variables. Correlations were detected between IMCL and protein-HNE. Conclusion: Our data show for the first time that skeletal muscle protein-HNE adducts are related to the severity of insulin resistance in sedentary adults. These results suggest that muscle lipid peroxidation could be involved in the development of insulin resistance. (J Clin Endocrinol Metab 97: E1182-E1186, 2012)
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