Journal
NEUROSCIENCE
Volume 295, Issue -, Pages 175-186Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuroscience.2015.03.037
Keywords
brain-derived neurotrophic factor; osmotic stress; cell swelling; glia; bipolar cell; retina
Categories
Funding
- Deutsche Forschungsgemeinschaft - Germany [GRK 1097/1, FOR 748, RE 849/16-1]
- Hans-Bockler Foundation - Federal Ministry of Education and Research - Germany (BMBF)
- CNPq - Brazil
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Water accumulation in retinal glial (Muller) and neuronal cells resulting in cellular swelling contributes to the development of retinal edema and neurodegeneration. Intravitreal administration of neurotrophins such as brain-derived neurotrophic factor (BDNF) is known to promote survival of retinal neurons. Here, we show that exogenous BDNF inhibits the osmotic swelling of Muller cell somata induced by superfusion of rat retinal slices or freshly isolated cells with a hypoosmotic solution containing barium ions. BDNF also inhibited the osmotic swelling of bipolar cell somata in retinal slices, but failed to inhibit the osmotic soma swelling of freshly isolated bipolar cells. The inhibitory effect of BDNF on Muller cell swelling was mediated by activation of tropomyosin-related kinase B (TrkB) and transactivation of fibroblast growth factor receptors. Exogenous basic fibroblast growth factor (bFGF) fully inhibited the osmotic swelling of Muller cell somata while it partially inhibited the osmotic swelling of bipolar cell somata. Isolated Muller cells displayed immunoreactivity of truncated TrkB, but not full-length TrkB. Isolated rod bipolar cells displayed immunoreactivities of both TrkB isoforms. Data suggest that the neuroprotective effect of exogenous BDNF in the retina is in part mediated by prevention of the cytotoxic swelling of retinal glial and bipolar cells. While BDNF directly acts on Muller cells by activation of TrkB, BDNF indirectly acts on bipolar cells by inducing glial release of factors like bFGF that inhibit bipolar cell swelling. (C) 2015 IBRO. Published by Elsevier Ltd. All rights reserved.
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