Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 951, Issue -, Pages 52-57Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2014.01.023
Keywords
Monoamine neurotransmitter; Ultra high performance liquid; chromatography; Microdialysis; Electrochemical detection
Funding
- Claude Bernard Lyon 1 University
- Centre de Recherche en Neurosciences de Lyon (CRNL)
- Centre National de la Recherche Scientifique (CNRS)
- Institut National de la Sante et de la Recherche Medicate (INSERM)
- Structure Federative de Recherche (SFR) Biosciences Gerland - Lyon Sud
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Electrochemical methods are very often used to detect catecholamine and indolamine neurotransmitters separated by conventional reverse-phase high performance liquid chromatography (HPLC). The present paper presents the development of a chromatographic method to detect monoamines present in low-volume brain dialysis samples using a capillary column filled with sub-2 mu m particles. Several parameters (repeatability, linearity, accuracy, limit of detection) for this new ultrahigh performance liquid chromatography (UHPLC) method with electrochemical detection were examined after optimization of the analytical conditions. Noradrenaline, adrenaline, serotonin, dopamine and its metabolite 3-methoxytyramine were separated in 1 mu l. of injected sample volume; they were detected above concentrations of 0.5-1 nmol/L, with 2.1-9.5% accuracy and intra-assay repeatability equal to or less than 6%. The final method was applied to very low volume dialysates from rat brain containing monoamine traces. The study demonstrates that capillary UHPLC with electrochemical detection is suitable for monitoring dialysate monoamines collected at high sampling rate. (C) 2014 Elsevier B.V. All rights reserved.
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