Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 966, Issue -, Pages 193-199Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2014.01.040
Keywords
Supercritical fluid chromatography; Regioisomers; Triacylglycerol; Triple quadrupole mass spectrometry
Funding
- MEXT KAKENHI [23686120]
- Development of Systems and Technology for Advanced Measurement and Analysis Project (JST)
- Advanced Low Carbon Technology Research and Development Program (JST)
- Grants-in-Aid for Scientific Research [24656503, 23686120] Funding Source: KAKEN
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In this study, supercritical fluid chromatography (SFC) coupled with triple quadrupole mass spectrometry was applied to the profiling of several regioisomeric triacylglycerols (TAGs). SFC conditions (column, flow rate, modifier) were optimized for the effective separation of TAGs. In the column test, a triacontyl (C30) silica gel reversed-phase column was selected to separate TAG regioisomers. Multiple reaction monitoring was used to selectively quantify each TAG. Then, the method was used to perform detailed characterization of a diverse array of TAGs in palm and canola oils. Seventy TAGS (C46:0-C60:2) of these oils were successfully analyzed as a result, and twenty isomeric TAG pairs were separated well. In particular, this method provided the fast and high resolution separation of six regioisomeric TAG pairs (PPLn/PLnP, PPL/PLP, PPO/POP, SPLn/SLnP, SPO/SOP, SSO/SOS-stearic acid (S, 18:0), oleic acid (0, 18:1), linoleic acid (L, 18:2), linolenic acid (Ln, 18:3), palmitic acid (P, 16:0)) in a short time (50 min) as compared to high performance liquid chromatography. We were able to demonstrate the utility of this method for the analysis of regioisomeric TAGs in edible oils. (C) 2014 Elsevier B.V. All rights reserved.
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