Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 962, Issue -, Pages 68-74Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2014.05.019
Keywords
Mycotoxins; Zearalenone; Zearalenone metabolites; UHPLC-MS/MS; Human urine samples; Tunisian women
Funding
- Regional Government of Andalusia [P09-CTS-4470, P09-CTS5488]
- Instituto de Salud Carlos III [CD012/00462]
- Ministry of Higher Education and Scientific Research of Tunisia
Ask authors/readers for more resources
Humans can be exposed to mycotoxins through the diet. Evaluation of exposure levels to mycotoxins can be performed by direct determination in urine. The present work proposes a sensitive ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method for the determination of zearalenone (ZON) and its five metabolites (alpha-zearalenolla [alpha-ZOL], beta-zearalenol [beta-ZOL], alpha-zearalanol [zeranol, alpha-ZAL], beta-zearalanol [teranol, beta-ZALI and zearalanone [ZAN]) in human urine samples. The method involves the enzymatic hydrolysis of the samples, extraction of the analytes using liquid-liquid extraction (LLE) with ethyl acetate/formic acid (99:1 v/v) and a cleanup step using hexane, prior to their quantification by UHPLC-MS/MS, using an electrospray ionization (ESI) interface in the negative mode. Zearalenone-d(6) (ZON-d(6)) was used as surrogate. The limits of detection and the limits of quantification ranged from 0.03 to 0.3 ng mL(-1) and from 0.1 to 1.0 ng mL(-1), respectively. The method was validated using matrix-matched calibration and a spike recovery assay. Recovery rates for spiked samples ranged from 96% to 104%, with relative standard deviations lower than 8.5%. This method was satisfactorily applied to 42 urine samples from Tunisian women for the determination of zearalenone and its five metabolites. (C) 2014 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available