Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 962, Issue -, Pages 1-8Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2014.05.014
Keywords
Cerbera manghas L.; Cardenolides; Forensic toxicology; Ultra-high performance liquid chromatography-photodiode array-mass spectrometry (UHPLC-PDA-MS); Ultra-high performance liquid chromatography-high resolution tandem mass spectrometry (UHPLC-HRMS/MS)
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The toxicity of the sea mango (Cerbera manghas L.) is well known. The plant is ranked as one of the deadliest of the southern Asian coastline. Cardenolidic heterosides are responsible for the cardiotoxicity of trees of the Cerbera genus. We have identified and determined the concentration of the principal glycosidic steroids present in the seeds of sea mangos (Thailand). Drug screening of an extract of the seeds was carried out using ultra-high performance liquid chromatography coupled to photodiode array detection and mass spectrometry (UHPLC-PDA-MS) with quantification at 219 nm. Identification was confirmed by UHPLC-HRMS. Deacetyltanghinin (m/z 549.3055 +/- 2 ppm), neriifolin (m/z 535.3259 +/- 2 ppm), tanghinin (m/z 591.3169 +/- 2 ppm) and cerberin (577.3375 +/- 2 ppm) were the most abundant glycosidic steroids present in the sea mango seeds. A seed of the dried ripe fruit had concentrations of 1209.1, 804.2, 621.4 and 285.9 mu g/g, respectively. A seed of the fresh unripe fruit had concentrations of 49.4, 47.0, 3.5 and 2.3 mu g/g. (C) 2014 Elsevier B.V. All rights reserved.
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