Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 944, Issue -, Pages 82-89Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2013.11.018
Keywords
Peperomia dindygulensis; Secolignans; Rat plasma; Pharmacokinetics; UFLC-MS/MS
Funding
- Natural Science Foundation of Jiangsu Province [BK20130954]
- Natural Science Foundation of Nanjing University of Chinese Medicines
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A rapid and sensitive ultra fast performance liquid chromatography-tandem mass spectrometry method was developed for the simultaneous determination of five bioactive secolignans in Peperomia dindygulensis extract, including peperomin A, peperomin B, peperomin C, 4 ''-hydroxypeperomin B and 4 ''-hydroxypeperomin C in rat plasma. Arctigenin was used as the internal standard. The separation was performed on an Innovation (TM) Polar-RP C-18 column by a gradient elution within a runtime of 7 min. The mobile phase consisted of A (methanol) and B (0.1% formic acid in water) at a flow rate of 0.4 mL/min. The detection was accomplished by using positive ion TurboIonSpray ionization in multiple reaction monitoring mode. The method was linear for all analytes over investigated range with all correlation coefficients greater than 0.9972. The lower limits of quantification were 1.1 ng/mL for peperomin A, 1.24 ng/mL for peperomin B, 1.02 ng/mL for peperomin C, 1.91 ng/mL for 4 ''-hydroxypeperomin B and 1.27 ng/mL for 4 ''-hydroxypeperomin C. The intra- and inter-day precision (RSD%) was within 15% and the accuracy (RE%) ranged from -11.7% to 10.3%. This simple and sensitive method was fully validated and successfully applied to the pharmacokinetic study of peperomin A, peperomin B, peperomin C, 4 ''-hydroxypeperomin B and 4 ''-hydroxypeperomin C in rat plasma after oral administration of P. dindygulensis extract. (C) 2013 Elsevier B.V. All rights reserved.
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