Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 917, Issue -, Pages 11-17Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2012.12.036
Keywords
Tetracyclines; Residues; Pressurized liquid extraction; Food of animal origin; HPLC-UV
Funding
- Research Foundation of Education Bureau of Hubei Province, China [Q20121801]
- Scientific Research Foundation of Wuhan Polytechnic University [2011388]
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A simple and especially rapid method, pressurized liquid extraction, has been developed and applied to the quantitative determination of oxytetracycline, tetracycline, chlortetracycline, minocycline, methacycline, demeclocycline and doxycycline in egg, fish and shrimp. The procedure consisted of a trichloracetic acid/methanol extraction conducted at elevated temperature (60 degrees C) and pressure (65 bar), without further clean-up, the extraction solution was concentrated and finally for high-performance liquid chromatography analysis. The limits of detection were 5.0-10.0 mu g/kg and the limits of quantification were 10.0-15.0 mu g/kg for tetracyclines in egg, fish and shrimp using UV detection. The analytical limits CC alpha and CC beta were also calculated. The recoveries of tetracyclines spiked at levels of 15-300 mu g/kg, averaged 75.6-103.5% with the relative standard deviation values less than 11%. The optimized procedure has been successfully applied to real samples in our laboratories. It demonstrated that the new method was robust and useful for monitoring and quantification of 7 tetracycline residues in food of animal origin. (c) 2013 Elsevier B.V. All rights reserved.
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