Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 927, Issue -, Pages 223-226Publisher
ELSEVIER
DOI: 10.1016/j.jchromb.2012.10.021
Keywords
2-Methylimidazole; 4-Methylimidazole; 2-Acetyl-4-(1,2,3,4-tetrahydroxybutyl)imidazole; Soft drinks; Caramel colours; HPLC-MS
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Substituted imidazoles recently came under scrutiny as they may be indirectly introduced into cola beverages via the use of class IV (E150d) caramel colours and may pose health hazards. A LC/MS/MS method was developed for determining 2- and 4-methylimidazole (2-MI, 4-MI) and 2-acetyl-4-(1,2,3,4)tetrahydroxybutylimidazole (THI) in beverages and caramel colours. The method is very rapid and easy to conduct as it requires only dilution in eluent for sample preparation. For 4-MI, the recovery was between 94 and 102% for spiked cola samples. The limit of detection was 2 mu g/L in the measuring solution (corresponding to 40 mu g/L for cola samples diluted 1:20 during sample preparation). 97 cola samples and 13 caramel colours from Germany and France were analysed. From the 3 analytes, only 4-MI was found in the samples with very varying concentrations (non quantifiable traces to 0.6 mg/L in colas and 175-658 mg/kg in E150d). The exposure for cola drinkers in worst case scenarios is estimated to be 2-5 mu g/kg bodyweight/day, which is judged as being only a low risk for public health. 2012 Elsevier B.V. All rights reserved.
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