Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 936, Issue -, Pages 88-94Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2013.06.037
Keywords
Sample preparation; Solid phase; Chelex resin; Flow-through polymerase chain reaction (PCR); Poly(methylmethacrylate) (PMMA); Integrated microdevice
Funding
- GRRC program of Gyeonggi Province (GRRC Gachon) [2013-B04]
- Public Welfare & Safety research program through the National Research Foundation of Korea (NRF)
- Ministry of Science, ICT and Future Planning [NRF-2012M3A2A1051681]
Ask authors/readers for more resources
In this study, we fabricate a monolithic poly(methylmethacrylate) (PMMA) microdevice on which solid phase-based DNA preparation and flow-through polymerase chain reaction (PCR) units were functionally integrated for one-step sample preparation and amplification operated by pressure. Chelex resin, which is used as a solid support for DNA preparation, can capture denatured proteins but releases DNA, and the purified DNA can then be used as a template in a subsequent amplification process. Using the PMMA microdevices, DNA was successfully purified from both Escherichia coli and human hair sample, and the plasmid vector inserted in E. coli and the D1S80 locus in human genomic DNA were successfully amplified from on-chip purified E. coli and human hair samples. Furthermore, the integration potential of the proposed sample preparation and flow-through PCR units was successfully demonstrate on a monolithic PMMA microdevice with a seamless flow, which could pave the way for a pressure-driven, simple one-step sample preparation and amplification with greatly decreased manufacture cost and enhanced device disposability. (C) 2013 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available