Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 942, Issue -, Pages 98-106Publisher
ELSEVIER
DOI: 10.1016/j.jchromb.2013.10.016
Keywords
Neutral lipids; Microalgae; HPLC; ELSD; APCI-MS/MS; SPE
Funding
- Total New Energy
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A method was developed to analyze neutral lipids through the use of three triglycerides, four free fatty acids, six di- and four mono-glycerides standards by high performance liquid chromatography (HPLC) normal phase coupled with either with evaporative light scattering detector (ELSD) or with mass spectrometry (MS) operating in atmospheric pressure chemical ionization (APCI) mode. The method was applied to the determination of the neutral lipid fraction from a Botryococcus braunii race A (B. braunii) culture. This method led us to identify neutral lipids synthesized by B. braunii in a single analysis within 45 min through HPLC-APCI-MS/MS technique. (C) 2013 Elsevier B.V. All rights reserved.
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