Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 927, Issue -, Pages 214-217Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2012.09.005
Keywords
4-Fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F); van Deemter equation; Biological sample; Fluorescence
Funding
- Grants-in-Aid for Scientific Research [23790041] Funding Source: KAKEN
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In this study, the separation efficiency of a core-shell particle column was compared with particle-packed and monolithic silica columns, which showed that the core-shell particle column had a smaller theoretical plate height and that its separation efficiency was not affected significantly by the increase in flow rate. A fast HPLC method using a core-shell particle column was developed for the determination of amino acids. 4-Fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) was used as a fluorescence derivatization reagent for amino acids, followed by separation on a core-shell Kinetex C18 column. The analysis time for 21 NBD-amino acids was within 7 min, which was faster than that in our previous studies with conventional particle-packed columns or monolithic silica columns. The linearities of the calibration curves for all the amino acids were found to be good over a range of injection amounts from 40 fmol to 40 pmol. The accuracies for the amino acid determinations were 90.9-107%. The method was proved to have potential for the fast determination of amino acids in biological samples. (C) 2012 Elsevier B.V. All rights reserved.
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