Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 901, Issue -, Pages 41-46Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2012.05.038
Keywords
GC x GC-TOFMS; SPME; Pseudomonas aeruginosa; Bacterial volatiles; Metabolomics
Funding
- National Center for Research Resources from the National Institutes of Health [5P20RR021905-07]
- National Institute of General Medical Sciences from the National Institutes of Health [8 P20 GM103496-07]
- Cystic Fibrosis Foundation [STANTO07R0]
- NASA [NNH09ZNE002C]
- National Science Foundation [0923295]
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [0923295] Funding Source: National Science Foundation
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Bacteria produce unique volatile mixtures that could be used to identify infectious agents to the species, and possibly the strain level. However, due to the immense variety of human pathogens, and the close relatedness of some of these bacteria, the robust identification of the bacterium based on its volatile metabolome is likely to require a large number of volatile compounds for each species. We applied comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC x GC-TOFMS) to the identification of the headspace volatiles of Pseudomonas aeruginosa PA14 grown for 24 h in lysogeny broth. This is the first reported use of GC x GC-TOFMS for the characterization of bacterial headspace volatiles. The analytical purity that is afforded by this chromatographic method facilitated the identification of 28 new P. aeruginosa-derived volatiles, nearly doubling the list of volatiles for this species. (C) 2012 Elsevier B.V. All rights reserved.
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