Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 908, Issue -, Pages 137-142Publisher
ELSEVIER
DOI: 10.1016/j.jchromb.2012.09.022
Keywords
Matrix solid-phase dispersion; Dummy imprinted polymers; Melamine; High performance liquid chromatography; Milk samples
Funding
- National Natural Science Foundation of China [20905019, 21011140338]
- Natural Science Foundation of Hebei [B2010000209]
- Science Foundation of Education Department of Hebei Province [CPRC003]
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A simple, convenient and high selective molecularly imprinted matrix solid-phase dispersion (MI-MSPD) using water-compatible cyromazine-imprinted polymer as adsorbent was proposed for the rapid screening of melamine from bovine milk coupled with liquid chromatography-ultraviolet detection. The molecularly imprinted polymers (MIPs) synthesized by cyromazine as dummy template and reformative methanol-water system as reaction medium showed higher affinity and selectivity to melamine, and so they were applied as the specific dispersant of MSPD to extraction of melamine and simultaneously eliminate the effect of template leakage on quantitative analysis. Under the optimized conditions, good linearity was obtained in a range of 0.24-60.0 mu g g(-1) with the correlation coefficient of 0.9994. The recoveries of melamine at three spiked levels were ranged from 86.0 to 96.2% with the relative standard deviation (RSD) <= 4.0%. This proposed MI-MSPD method combined the advantages of MSPD and MIPs, and could be used as an alternative tool for analyzing the residues of melamine in complex milk samples. (C) 2012 Elsevier B.V. All rights reserved.
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