EFFECTS OF ANGIOTENSIN II ON LEPTIN AND DOWNSTREAM LEPTIN SIGNALING IN THE CAROTID BODY DURING ACUTE INTERMITTENT HYPOXIA

Angiotensin II (ANG II) is known to promote leptin production and secretion. Although ANG II type 1 receptors (AT(1)Rs) and leptin are expressed within the carotid body, it is not known whether AT(1)R and leptin are co-expressed in the same glomus cells nor if these peptides are affected within the carotid body by intermittent hypoxia (IH). This study was done to investigate whether ANG II modulated leptin signaling in the carotid body during IH. Rats were treated with captopril (Capt) or the AT(1)R blocker losartan (Los) in the drinking water for 3 days prior to being exposed to IH (8h) or normoxia (8h). IH induced increases in plasma ANG II and leptin compared to normoxic controls. Capt treatment abolished the plasma leptin changes to IH, whereas Los treatment had no effect on the IH induced increase in plasma leptin. Additionally, carotid body glomus cells containing both leptin and the long form of the leptin receptor (OB-Rb) were found to co-express AT(1)R protein, and IH increased the expression of only AT(1)R protein within the carotid body in both Capt-and non-Capt-treated animals. On the other hand, Los treatment did not modify AT(1)R protein expression to IH. Additionally, Capt and Los treatment eliminated the elevated carotid body leptin protein expression, and the changes in phosphorylated signal transducer and activator of transcription three protein, the short form of the leptin receptor (OB-R100), suppressor of cytokine signaling 3, and phosphorylated extracellular-signal-regulated kinase 1/2 protein expression induced by IH. However, Capt elevated the expression of OB-Rb protein, whereas Los abolished the changes in OB-Rb protein to IH. These findings, taken together with the previous observation that ANG II modifies carotid body chemosensitivity, suggest that the increased circulating levels of ANG II and leptin induced by IH act at the carotid body to alter leptin signaling within the carotid body which in turn may influence chemoreceptor function. (C) 2015 IBRO. Published by Elsevier Ltd. All rights reserved.