Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 898, Issue -, Pages 95-100Publisher
ELSEVIER
DOI: 10.1016/j.jchromb.2012.04.024
Keywords
Rutin; Intestinal bacteria; UPLC-Q-TOF/MS; Deglycosylation; Enzymic activity
Funding
- Jiangsu province colleges and universities [10KJA360039]
- Construction Project for Jiangsu Key Laboratory for High Technology Research of TCM Formulae [BM2010576]
- National Basic Research Program of China (973 Program) [2011CB505300, 2011CB505303]
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In this paper, rutin was metabolized by human intestinal bacteria and five isolated strains including Bacillus sp. 52, Bacteroides sp. 45, 42, 22 and Veillonella sp. 32. the metabolites were identified using ultra performance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF/MS). As a result. Bacillus sp. 52 and Bacteroides sp. 45 could metabolize rutin to quercetin 3-O-glucoside and leucocyanidin. Bacteroides sp. 42 and Veillonella sp. 32 could convert rutin to leucocyanidin. Bacteroides sp. 22 could hydrolyze rutin to quercetin-3-O-glucoside. In order to further explain the metabolism pathway of rutin, the beta-D-glucosidase and alpha-L-rhamnosidase activities of five strains were determined. Bacteroides sp. 22 could produce alpha-L-rhamnosidase but did not produce beta-D-glucosidase or beta-D-glucosidase activity was too low to be detected. The other four strains all demonstrated alpha-L-rhamnosidase and beta-D-glucosidase activities. Furthermore, alpha-L-rhamnosidase and beta-D-glucosidase activities of Veillonella sp. 32 and Bacteroides sp. 42 were higher than those of Bacteroides sp. 45 and Bacillus sp. 52. Based on these results, we can propose the deglycosylated rout of rutin: rutin was metabolized to be quercetin-3-O-glucoside by alpha-L-rhamnosidase produced from these bacteria, thereafter, quercetin-3-O-glucoside was further metabolized by beta-D-glocosidase to form leucocyanidin. Because of the higher enzyme activity in Veillonella sp. 32 and Bacteroides sp. 42, quercetin-3-O-glucoside was completely metabolized to leucocyanidin by these two bacteria. Due to the lack of beta-D-glucosidase activity, Bacteroides sp. 22 could not further metabolize quercetin-3-O-glucoside to leucocyanidin. This study will be helpful for understanding the deglycosylated rout of rutin and the role of different intestinal bacteria on the metabolism of natural compounds. (C) 2012 Elsevier B.V. All rights reserved.
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