Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 883, Issue -, Pages 68-75Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2011.10.018
Keywords
Clinical application; Metabolomics; LC-MS/MS; Tandem mass spectrometry
Funding
- LIFE - Leipzig Research Center for Civilization Diseases, University Leipzig
- European Union
- European Regional Development Fund (ERDF)
- Free State of Saxony
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The analysis of metabolites in human body fluids remains a challenge because of their chemical diversity and dynamic concentration range. Liquid chromatography (LC) in combination with tandem mass spectrometry (MS/MS) offers a robust, reliable, and economical methodology for quantitative single metabolite analysis and profiling of complete metabolite classes of a biological specimen over a broad dynamic concentration range. The application of LC-MS/MS based metabolomic approaches in clinical applications aims at both, the improvement of diagnostic sensitivity and specificity by profiling a metabolite class instead of a single metabolite analysis, and the identification of new disease specific biomarkers. In the present paper we discuss recent advances in method development for LC-MS/MS analysis of lipids, carbohydrates, amino acids and biogenic amines, vitamins and organic acids with focus on human body fluids. In this context an overview on recent LC-MS/MS based metabolome studies for cancer, diabetes and coronary heart disease is presented. (C) 2011 Elsevier B.V. All rights reserved.
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